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Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area

By Liliane Maria Vidal Siqueira, Luciana Inácia Gomes, Edward Oliveira, Eduardo Ribeiro de Oliveira, Áureo Almeida de Oliveira, Martin Johannes Enk, Nídia Figueiredo Carneiro, Ana Rabello and Paulo Marcos Zech Coelho


This study evaluated parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni. A population-based study was performed in 201 inhabitants from a low transmission locality named Pedra Preta, municipality of Montes Claros, state of Minas Gerais, Brazil. Four stool samples were analysed using two techniques, the Kato-Katz® (KK) technique (18 slides) and the TF-Test®, to establish the infection rate. The positivity rate of 18 KK slides of four stool samples was 28.9% (58/201) and the combined parasitological techniques (KK+TF-Test®) produced a 35.8% positivity rate (72/201). Furthermore, a polymerase chain reaction (PCR)-ELISA assay produced a positivity rate of 23.4% (47/201) using the first sample. All 72 patients with positive parasitological exams were treated with a single dose of Praziquantel® and these patients were followed-up 30, 90 and 180 days after treatment to establish the cure rate. Cure rates obtained by the analysis of 12 KK slides were 100%, 100% and 98.4% at 30, 90 and 180 days after treatment, respectively. PCR-ELISA revealed cure rates of 98.5%, 95.5% and 96.5%, respectively. The diagnostic and assessment of cure for schistosomiasis may require an increased number of KK slides or a test with higher sensitivity, such as PCR-ELISA, in situations of very low parasite load, such as after therapeutic interventions

Topics: Schistosoma mansoni, schistosomiasis, diagnosis, control, low transmission area, Brazil, Arctic medicine. Tropical medicine, RC955-962, Microbiology, QR1-502
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Year: 2015
DOI identifier: 10.1590/0074-02760140375
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