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Characterisation of PLD activity in real-time

By Mina-Olga Aletrari


PLD catalyses hydrolysis of phosphatidylcholine (PtdCho) to produce phosphatidic acid\ud (PtdOH) and choline. PtdOH is a second messenger responsible for a multitude of cell\ud processes, ranging from cytoskeletal rearrangement to cell proliferation. Antigenic\ud stimulation of RBL-2H3 mast cells and growth factor stimulation of endothelial HeLa\ud cells results in PLD-dependent exocytosis and endocytosis, respectively. A novel\ud fluorescent PtdCho (fPtdCho) was used to label both cell lines and Bligh-Dyer lipid\ud extraction of fPtdCho-labelled RBL-2H3 cells showed the lipid was intact post-labelling.\ud fPtdCho co-localised up to 50% with the lysosomal marker LysoTracker Red in RBL-\ud 2H3 cells, and was not secreted in response to antigenic stimulation as recorded using\ud real-time confocal microscopy. Primary alcohol treatment of fPtdCho-labelled RBL-\ud 2H3 cells altered fPtdCho-labelling to diffuse from punctate distribution, suggesting\ud PLD-generated PtdOH is responsible for retention of punctate fPtdCho staining. PLD\ud isoforms 1b and 2a were labelled with Cherry (a red fluorescent protein) and transiently\ud expressed in fPtdCho-labelled HeLa cells. Localisation was assessed using FRET by\ud FRAP technology in live cells and showed that substrate and lipase were in close\ud proximity. These findings will facilitate future development of a live real-time in vivo\ud PLD assay. Furthermore, localisation of PLD and its activator Rac1 was assessed at rest\ud and in EGF-stimulated HeLa cells in real-time. This showed co-localisation between\ud PLD and Rac1 following stimulation. The fluorescent PtdCho was also used to develop\ud a novel real-time in vitro PLD assay, monitoring fPtdCho metabolism at two second\ud intervals. This in vitro assay is more sensitive than traditional end-point assays and will\ud help clarify the relative rate of PLD activation in response to small G-protein activators\ud and other co-factors in real-time

Topics: QP
OAI identifier: oai:wrap.warwick.ac.uk:4479

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  1. (1999). 3T3-L1 adipocytes express two isoforms of phospholipase D in distinct subcellular compartments.
  2. (2009). 5-fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis.
  3. (2002). A conserved C-terminal domain of EFA6-family ARF6-guanine nucleotide exchange factors induces lengthening of microvillilike membrane protrusions.
  4. (2003). A decade of caspases.
  5. (1994). A fluorescent assay for agonist-activated phospholipase D in mammalian cell extracts.
  6. (2005). A guide to choosing fluorescent proteins.
  7. (1996). A human exchange factor for ARF contains Sec7-and pleckstrin-homology domains.
  8. (1994). A mammalian protein targeted by G1-arresting rapamycin-receptor complex.
  9. (2002). A monomeric red fluorescent protein.
  10. (2006). A mutation in Epstein-Barr virus LMP2A reveals a role for phospholipase D in B-Cell antigen receptor trafficking.
  11. (1990). A neutral phospholipase D activity from rat brain synaptic plasma membranes. Identification and partial characterization.
  12. (1996). A novel family of phospholipase D homologues that includes phospholipid synthases and putative endonucleases: identification of duplicated repeats and potential active site residues.
  13. (2000). A pleckstrin homology domain specific for phosphatidylinositol 4, 5-bisphosphate (PtdIns-4,5-P2) and fused to green fluorescent protein identifies plasma membrane PtdIns-4,5-P2 as being important in exocytosis.
  14. (2002). A real-time fluorogenic phospholipase A2 assay for biochemical and cellular activity measurements.
  15. (2000). A role for phospholipase D in GLUT4 glucose transporter translocation.
  16. (1999). A role for rho-kinase in rho-controlled phospholipase D stimulation by the m3 muscarinic acetylcholine receptor.
  17. (2008). A systematic RNAi screen reveals involvement of endocytic pathway in neuronal dysfunction in alpha-synuclein transgenic C.
  18. (1988). A view of acidic intracellular compartments.
  19. (1972). A23187: A divalent cation ionophore.
  20. (1995). Accumulation of phosphatidylalcohol in cultured cells: use of subcellular fractionation to investigate phospholipase D activity during signal transduction.
  21. (2004). Acidic vesicles in living hyphae of an arbuscular mycorrhizal fungus, Gigaspora margarita. Plant Soil.
  22. (2001). Actin directly interacts with phospholipase D, inhibiting its activity.
  23. (1993). Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts.
  24. (1990). Activation of human neutrophil phospholipase D by three separable mechanisms.
  25. (1995). Activation of pancreatic acinar cell phospholipase D by epidermal, insulin-like, and basic fibroblast growth factors involves tyrosine kinase.
  26. (1994). Activation of phospholipase Cgamma is necessary for stimulation of phospholipase D by platelet-derived growth factor.
  27. (1996). Activation of phospholipase D and phosphatidylinositol 4-phosphate 5-kinase in HL60 membranes is mediated by endogenous Arf but not Rho.
  28. (1994). Activation of phospholipase D induced by platelet-derived growth factor is dependent upon the level of phospholipase C-gamma 1.
  29. Activation of phospholipase D is not mediated by direct phosphorylation on tyrosine residues.
  30. (2000). Activation of phospholipase D1 by Cdc42 requires the Rho insert region.
  31. (1994). Activation of rat brain phospholipase D by ADP-ribosylation factors 1, 5, and 6: Separation of ADP-ribosylation factor-dependent and oleatedependent enzymes.
  32. (2007). Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains.
  33. (1999). ADP ribosylation factor 1 mutants identify a phospholipase D effector region and reveal that phospholipase D participates in lysosomal secretion but is not sufficient for recruitment of coatomer I.
  34. (2003). ADP-ribosylation factor 6 regulates insulin secretion through plasma membrane phoshatidylinositol 4,5-bisphosphate.
  35. (1993). ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity.
  36. (2008). Alpha-actinin structure and regulation.
  37. (2002). alpha-Synuclein interacts with phospholipase D isozymes and inhibits pervanadate-induced phospholipase D activation in human embryonic kidney-293 cells.
  38. (2009). Amphiphysin I and regulation of synaptic vesicle endocytosis.
  39. (1990). An indirect pathway of receptor-mediated 1,2-diacylglycerol formation in mast cells. I. IgE receptormediated activation of phospholipase D.
  40. (2002). Antigen-stimulated activation of phospholipase D1b by
  41. (1999). AP-2 recruitment to synaptotagmin stimulated by tyrosine-based endocytic motifs.
  42. Apoptosis and alternative deathstyles.
  43. (1999). Apoptosis and the skin.
  44. (2007). Apoptosis: A Review of Programmed Cell Death.
  45. (1995). Arf proteins: the membrane traffic police? Trends Biochem.
  46. (1998). Association of N- and C-terminal domains of phospholipase D is required for catalytic activity.
  47. (2000). Association of the N- and C-terminal domains of phospholipase D. Contribution of the conserved HKD motifs to the interaction and the requirement of the association for Ser/Thr phosphorylation of the enzyme.
  48. (2000). Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.
  49. (1992). Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein.
  50. (1992). Brefeldin A: insights into the control of membrane traffic and organelle structure.
  51. (2002). Calcium-regulated exocytosis of dense-core vesicles requires the activation of ADP-ribosylation factor (ARF)6 by ARF nucleotide binding site opener at the plasma membrane.
  52. (2000). Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor-reversible manner.
  53. (1998). Caveolins, a family of scaffolding proteins for organizing "preassembled signaling complexes" at the plasma membrane.
  54. (1996). Cellpermeable ceramides prevent the activation of phospholipase D by ADP-ribosylation factor and RhoA.
  55. (2001). Ceramide inhibition of mammalian phospholipase D1 and D2 activities is antagonized by phosphatidylinositol 4,5-bisphosphate.
  56. (1996). Ceramide inhibits IgE-mediated activation of phospholipase D, but not of phospholipase C, in rat basophilic leukemia (RBL-2H3) cells.
  57. (1996). Ceramide inhibits phospholipase D in a cell-free system.
  58. (2004). Ceramide kinase is a mediator of calcium-dependent degranulation in mast cells.
  59. (1997). Characteristics of protein-kinase-C- and ADP-ribosylation-factor-stimulated phospholipase D activities in human embryonic kidney cells.
  60. (1998). Characterization of a rat brain phospholipase D isozyme.
  61. (2005). Characterization of exosome subpopulations from RBL-2H3 cells using fluorescent lipid. Blood Cells Mol.
  62. (1998). Characterization of human PLD2 and the analysis of PLD isoform splice variants.
  63. (2003). Characterization of the membrane binding mode of the C2 domain of PKC epsilon.
  64. (1999). Characterization of the regulation of phospholipase D activity in the detergentinsoluble fraction of HL60 cells by protein kinase C and small G-proteins.
  65. (1997). Characterization of two alternatively spliced forms of phospholipase D1: Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric GTP-binding proteins and protein kinase C-ʱ.
  66. (1990). Chelerythrine is a potent and specific inhibitor of protein kinase
  67. (1981). Choline metabolism and phosphatidylcholine biosynthesis in cultured rat hepatocytes.
  68. (1998). Cloning and initial characterization of a human phospholipase D2 (hPLD2). ADP-ribosylation factor regulates hPLD2.
  69. (1998). Cloning, differential regulation and tissue distribution of alternatively spliced isoforms of 302 ADP-ribosylation-factor-dependent phospholipase D from rat liver.
  70. (1999). Colocalization of phospholipase D1 and GTP-binding-defective mutant of ADPribosylation factor 6 to endosomes and lysosomes.
  71. (1999). Concerted action of cytosolic Ca 2+ and protein kinase C in receptor-mediated phospholipase D activation in Chinese hamster ovary cells expressing the cholecystokinin-A receptor.
  72. (2000). Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae.
  73. (2002). Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells.
  74. (1993). Correlation between secretion and phospholipase D activation in differentiated HL60 cells.
  75. (1999). Coupled inositide phosphorylation and phospholipase D activation initiates clathrin-coat assembly on lysosomes.
  76. (1999). Crystal structure of a phospholipase D family member.
  77. (1998). Cutting edge: Ca 2+-dependent exocytosis in mast cells is stimulated by the Ca 2+ sensor, synaptotagmin I.
  78. (2002). Cyclic nucleotide research - still expanding after half a century.
  79. (1997). Cytohesin1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor.
  80. (1994). Cytotoxic effects of chimeric protein consisting of tetanus toxin light chain and anthrax toxin lethal factor in non-neuronal cells.
  81. (1998). Definition of the protein kinase C interaction site of phospholipase D.
  82. (2003). Degranulation in RBL-2H3 cells: regulation by calmodulin pathway.
  83. (1991). Demethoxyviridin and wortmannin block phospholipase C and D activation in the human neutrophil.
  84. (2009). Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.
  85. (2002). Detecting proteinphospholipid interactions: Epidermal growth factor-induced activation of phospholipase D1b in situ.
  86. (2009). Determination of EGFR endocytosis kinetic by auto-regulatory association of PLD1 with µ2. PLoS One.
  87. (2001). Determination of interaction sites of phospholipase D1 for RhoA.
  88. (1998). Determination of interaction sites on the small G protein RhoA for phospholipase D.
  89. (1998). Diacylglycerols and phosphatidates: which molecular species are intracellular messengers? Trends Biochem.
  90. (1998). Differential regulation of phospholipase A2 (PLA2)-dependent Ca 2+ signaling in smooth muscle by cAMP- and cGMPdependent protein kinases. Inhibitory phosphorylation of PLA2 by cyclic nucleotidedependent protein kinases.
  91. (1982). Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
  92. (1994). Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus.
  93. (2000). Dual requirement for Rho and protein kinase C in direct activation of phospholipase D1 Through G protein-coupled receptor signaling.
  94. (2002). Dual role for phosphoinositides in regulation of yeast and mammalian phospholipase D enzymes.
  95. (1995). EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif.
  96. (1999). EFA6, a sec7 domain-containing exchange factor for ARF6, coordinates membrane recycling and actin cytoskeleton organization.
  97. (1997). Effects of brefeldin A on phosphatidylcholine phospholipase D and inositolphospholipid metabolism in HL-60 cells.
  98. (2001). Effects of cytochalasin D and lactrunculin B on mechanical properties of cells.
  99. (2003). Effects of Cytochalasin D on the actin cytoskeleton: association of neoformed actin aggregates with proteins involved in signalling and endocytosis.
  100. (2001). Effects of Latrunculin reveal requirements for the actin cytoskeleton during secretion from mast cells.
  101. (2003). Elevated phospholipase D activity in H-Ras- but not KRas-transformed cells by the synergistic action of RalA and ARF6.
  102. (2003). Endogenous phospholipase D2 localizes to the plasma membrane of RBL-2H3 mast cells and can be distinguished from ADP ribosylation factor-stimulated phospholipase D1 activity by its specific sensitivity to oleic acid.
  103. (2001). Endosomal localization of phospholipase D1a and 1b is defined by the C-termini of the proteins, and is independent of activity.
  104. (1991). Endothelin-1 activates phospholipase D and thymidine incorporation in fibroblasts overexpressing protein kinase C beta 1. Cell Regul.
  105. (1995). Enhanced hydrolysis of phosphatidylcholine by human group II non-pancreatic secreted phospholipase A2 as a result of interfacial activation by specific anions. Potential role of cholesterol sulphate.
  106. (1992). Epidermal growth factor increases sn-1,2-diacylglycerol levels and activates phospholipase D-catalysed phosphatidylcholine 293 breakdown in Swiss 3T3 cells in the absence of inositol-lipid hydrolysis.
  107. (1991). Epidermal growth factor-induced hydrolysis of phosphatidylcholine by phospholipase D and phospholipase C in human dermal fibroblasts.
  108. (1990). Epidermal growth factor.
  109. (1987). Essential synergy between Ca 2+ and guanine nucleotides in exocytotic secretion from permeabilized rat mast cells.
  110. (1990). Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms.
  111. (1995). Evidence for ADP-ribosylation-factor-mediated activation of phospholipase D by m3 muscarinic acetylcholine receptor.
  112. (1996). Evidence that phospholipase D mediates ADP ribosylation factor- dependent formation of Golgi coated vesicles.
  113. (2010). Exocytotic vesicle behaviour assessed by total internal reflection fluorescence microscopy.
  114. (2008). Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15. Protein Expr.
  115. (2004). Expression of dominant negative rab5 in HeLa cells regulates endocytic trafficking distal from the plasma membrane.
  116. (1999). Expression of phospholipase D isoforms in mammalian cells.
  117. (1999). Fatty acylation of phospholipase D1 on cysteine residues 240 and 241 determines localization on intracellular membranes.
  118. (2002). FcεRI crosslinking-induced actin assembly mediates calcium signalling in RBL-2H3 mast cells.
  119. (2000). Finding nuclear localization signals.
  120. (2008). Flexibility of aromatic residues in the active-site gorge of acetylcholinesterase: X-ray versus molecular dynamics.
  121. (1975). Fluorescence quenching of phenolic antioxidants and selective determination of propyl gallate.
  122. (2005). Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching.
  123. (1998). Fodrin inhibits phospholipases A2, C, and D by decreasing polyphosphoinositide cell content.
  124. (2003). Functional analysis of a phosphatidic acid binding domain in human Raf-1 kinase: mutations in the phosphatidate binding domain lead to tail and trunk abnormalities in developing zebrafish embryos.
  125. (2002). Functional roles of lipids in membranes.
  126. (2008). GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA.
  127. (2007). GEFs and GAPs: Critical elements in the control of small G proteins.
  128. (1998). Genomic analysis of murine phospholipase D1 and comparison to phospholipase D2 reveals an unusual difference in gene size.
  129. (2004). guanine nucleotide exchange factor ARNO-Sec7.
  130. (2004). Heart Circ.
  131. (2006). Helix-1 of the cAMP-specific phosphodiesterase PDE4A1 regulates its phospholipase-D-dependent redistribution in response to release of Ca 2+.
  132. (2009). Hexosaminidase assays.
  133. (2002). Hierarchy of membrane-targeting signals of phospholipase D1 involving lipid modification of a pleckstrin homology domain.
  134. (1997). High rates of actin filament turnover in budding yeast and roles for actin establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.
  135. (2001). How proteins move lipids and lipids move proteins.
  136. (1995). Human ADPribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family.
  137. (2004). Human cardiac phospholipase D activity is tightly controlled by phosphatidylinositol 4,5-bisphosphate.
  138. (1984). Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.
  139. (1997). Human phospholipase D1 can be tyrosine-phosphorylated in HL-60 granulocytes.
  140. (1992). Hydrolysis of phosphatidylcholine is stimulated by Ras proteins during mitogenic signal transduction.
  141. (1997). Identification of a novel Ca 2+-dependent, phosphatidylethanolamine hydrolyzing phospholipase D in yeast bearing a disruption in PLD1.
  142. (1996). Identification of a novel, Ca 2+-dependent phospholipase D with preference for phosphatidylserine and phosphatidylethanolamine in Saccharornyces cerevisiae.
  143. (1996). Identification of a phosphatidylinositol 4,5-bisphosphate-binding site in chicken skeletal muscle alphaactinin.
  144. (1999). Identification of a phosphoinositide binding motif that mediates activation of mammalian and yeast phospholipase D isoenzymes.
  145. (1995). Identification of an 11-kDa FKBP12-rapamycin-binding domain within the 289-kDa FKBP12-rapamycin-associated protein and characterization of a critical serine residue.
  146. (2008). Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a.
  147. (1998). Identification of lipid inhibitor of mammalian phospholipase
  148. (1980). Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
  149. (1996). Imaging exocytosis and endocytosis.
  150. (2004). Imaging phosphorylation dynamics of the epidermal growth factor receptor.
  151. (2007). Imaging proteins in vivo using fluorescence lifetime microscopy.
  152. (1982). Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae.
  153. (2009). Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P2.
  154. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein.
  155. Increased activity of small GTP-binding protein-dependent phospholipase D during differentiation in human promyelocytic leukemic HL60 cells.
  156. (2000). Independent functioning of cytosolic phospholipase A2 and phospholipase D1 in Trp-Lys-Tyr-Met-Val-D-Met-induced superoxide generation in human monocytes.
  157. Induction of the high-affinity IgE receptor (FcεRI) on human mast cells by IL-4.
  158. (2000). Influence of phosphtidylinositol 4,5-bisphosphate on human phospholipase D1 wild-type and deletion mutants: is there evidence for an interaction of phosphatidylinositol 4,5-bisphosphate with the putative pleckstrin homology domain?
  159. (2007). Inhibition of de novo ceramide synthesis upregulates phospholipase D and enhances myogenic differentiation.
  160. (2005). Inhibition of muscarinic receptor-linked phospholipase D activation by association with tubulin.
  161. (2000). Inhibition of phosphatidic acid synthesis alters the structure of the Golgi apparatus and inhibits secretion in endocrine cells.
  162. (1996). Inhibition of phospholipase D activity by fodrin. An active role for the cytoskeleton.
  163. (2000). Inhibition of phospholipase D by amphiphysins.
  164. (1997). Inhibition of phospholipase D by clathrin assembly protein 3 (AP3).
  165. (1997). Inhibition of phospholipid signalling and proliferation of Swiss 3T3 cells by the wortmannin analogue demethoxyviridin.
  166. (1989). Inhibition studies on the membrane-associated phospholipase A2 in vitro and prostaglandin E2 production in vivo of the macrophage-like P388D1 cell. Effects of manoalide, 7,7-dimethyl-5,8-eicosadienoic acid, and p-bromophenacyl bromide.
  167. (2002). Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures.
  168. (2000). Insulin-induced phospholipase D1 and phospholipase D2 activity in human embryonic kidney-293 cells mediated by the phospholipase C gamma and protein kinase C alpha signalling cascade.
  169. (2001). Interaction of phospholipase D1 with a casein-kinase-2-like serine kinase.
  170. (2007). Interaction of PLD1b with actin in antigen-stimulated mast cells.
  171. (1999). Interaction of the small G protein RhoA with the C terminus of human phospholipase D1.
  172. (2000). Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity.
  173. (2003). Intracellular delivery of bioactive peptides to RBL-2H3 cells induces β-hexosaminidase secretion and phospholipase D activation.
  174. (2001). Intracellular localization of phospholipase D1 in mammalian cells.
  175. (1982). Introduction, definitions, order of reaction and rate constants.
  176. (1998). Involvement of tyrosine phosphorylation and protein kinase C in the activation of phospholipase D by H2O2 in Swiss 3T3 fibroblasts.
  177. (2010). Key factors in mTOR regulation.
  178. (2006). Kinetic analysis of a mammalian phospholipase D: allosteric modulation by monomeric GTPases, protein kinase C, and polyphosphoinositides.
  179. (2001). Lipid activation of CTP:phosphocholine cytidylyltransferase ʱ: characterization and identification of a second activation domain.
  180. (1999). Lipid regulators of membrane traffic through the Golgi complex.
  181. (2005). Localization and function of Arf family GTPases.
  182. (2005). Localization and regulation of phospholipase D2 by ARF6.
  183. (1990). Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
  184. (2002). Localization of phospholipase D1 to caveolin-enriched membrane via palmitoylation: Implications for epidermal growth factor signaling.
  185. (1997). Lysophosphatidic acid induces threonine phosphorylation of Tiam1 in Swiss 3T3 fibroblasts via activation of protein kinase
  186. (1999). Mammalian phospholipase D structure and regulation.
  187. (2008). Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms.
  188. (1998). Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.
  189. (2002). Mechanism of ADP ribosylation factor-stimulated phosphatidylinositol 4,5-bisphosphate synthesis in HL60 cells.
  190. (2003). Mechanisms of regulation of phospholipase D1 by protein kinase
  191. (2008). Mechanistic analysis of the amplification and diversification events induced by Vav proteins in B-lymphocytes.
  192. (2006). Membrane and juxtamembrane targeting by PH and PTB domains.
  193. (2004). Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase.
  194. (2004). Membrane lipids and vesicular traffic.
  195. (2008). Membrane recognition by phospholipid-binding domains.
  196. (2003). Membrane topography of human phosphatidylethanolamine N-methyltransferase.
  197. (1996). Messenger functions of phosphatidic acid.
  198. (1997). Metal ion stimulation of phospholipase D-like activity of isolated rat intestinal mitochondria.
  199. (2004). Microtubules as a target for anticancer drugs.
  200. (2000). Mitogenic phospholipase D activity is restricted to caveolinenriched membrane microdomains.
  201. (1974). Mitogenic properties of a calcium ionophore, A23187.
  202. (1999). Modification of catalytically active phospholipase D1 with fatty acid in vivo.
  203. (2003). Modulation of membrane curvature of phosphatidic acid and lysophosphatidic acid.
  204. (2009). Modulation of phospholipase D activity in vitro.
  205. (2005). Modulation of the mammalian target of rapamycin pathway by diacylglycerol kinase-produced phosphatidic acid.
  206. (1999). Molecular analysis of mammalian phospholipase D2.
  207. (2003). Molecular cloning of a phospholipase D gene from Aspergillus nidulans and characterization of its deletion mutants.
  208. (2000). Molecular mechanisms of UV-induced apoptosis.
  209. (2006). Monomeric red fluorescent protein variants used for imaging studies in different species.
  210. (2006). Motion matters: Secretory granule motion adjacent to the plasma membrane and exocytosis.
  211. (1997). Mutagenesis of phospholipase D defines a superfamily including a trans-Golgi viral protein required for poxvirus pathogenicity.
  212. (2002). Networking of phospholipases in plant signal transduction.
  213. (1997). New developments in phospholipase
  214. (2009). NLStradamus: a simple Hidden Markov Model for nuclear localization signal prediction.
  215. (2001). Normal and abnormal secretion by haemopoietic cells.
  216. (1996). Novel domains in NADPH oxidase subunits, sorting nexins, and PtdIns 3-kinases: binding partners of SH3 domains? Protein Sci.
  217. (1994). Novel function of phosphatidylinositol 4,5-bisphosphate as a cofactor for brain membrane phospholipase D.
  218. (2005). Novel functions of the phospholipase D2-phox homology domain in protein kinase Cδ activation.
  219. (2002). Novel mastroparan analogs induce differential secretion from mast cells.
  220. (1995). Nuclear phospholipase D in Madin-Darby canine kidney cells. Guanosine 5′-O-(thiotriphosphate)-stimulated activation is mediated by RhoA and is downstream of protein kinase
  221. (2006). On the effects of site-specific mutations on activity and expression of the Streptomyces PMF phospholipase
  222. (1970). On the mechanism of enzymatic phosphatidylation. Biosynthesis of cardiolipin catalyzed by phospholipase D.
  223. (2002). P-selectin targeting to secretory lysosomes of RBL-2H3 cells.
  224. (1993). Pancreatic beta cell line MIN6 exhibits characteristics of glucose metabolism and glucose-stimulated insulin secretion similar to those of normal islets.
  225. (1995). Partial purification and characterization of Arf-sensitive phospholipase D from porcine brain.
  226. (2003). PDE4 cAMP phosphodiesterases: modular enzymes that orchestrate signalling cross-talk, desensitization and compartmentalization.
  227. (2002). Pharmacological importance of phospholipase D and phosphatidic acid in the regulation of the mitogen-activated protein kinase cascade.
  228. (1997). Phosphatase inhibitors potentiate 4-hydroxynonenal-induced phospholipase D activation in vascular endothelial cells.
  229. (2007). Phosphatidic acid domains in membranes: Effect of divalent counterions.
  230. (1992). Phosphatidic acid is a specific activator of phosphatidylinositol-4-phosphate kinase.
  231. (2010). Phosphatidic acid plays a regulatory role in clathrin-mediated endocytosis.
  232. (1992). Phosphatidic acid that accumulates in platelet-derived growth factor-stimulated Balb/c 3T3 cells is a potential mitogenic signal.
  233. (2006). Phosphatidic acid- and phosphatidylserinebinding proteins.
  234. Phosphatidic acid-mediated mitogenic activation of mTOR signaling.
  235. (2008). Phosphatidylcholine and choline homeostasis.
  236. (2005). Phosphatidylinositol (3,4,5)-trisphosphate specifically interacts with the phox homology domain of phospholipase D1 and stimulates its activity.
  237. (1999). Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation.
  238. (2003). Phosphohydrolase and transphosphatidylation reactions of two Streptomyces phospholipase D enzymes: Covalent versus noncovalent catalysis.
  239. (2005). Phosphoinositide regulation of clathrin-mediated endocytosis.
  240. (1990). Phospholipase Cmediated hydrolysis of phosphatidlycholine is an important step in PDGF-stimulated DNA synthesis.
  241. (1991). Phospholipase D activation in a cell-free system from human neutrophils by phorbol 12-myristate 13-acetate and guanosine 5'-O-(3-thiotriphosphate). Activation is calcium dependent and requires protein factors in both the plasma membrane and cytosol.
  242. (2008). Phospholipase D activity regulates integrinmediated cell spreading and migration by inducing GTP-Rac translocation to the plasma membrane.
  243. (2002). Phospholipase D and immune receptor signalling.
  244. (1999). Phospholipase D and its product, phosphatidic acid, mediate agonist-dependent raf-1 translocation to the plasma membrane and the activation of the mitogen-activated protein kinase pathway.
  245. (2009). Phospholipase D in endocytosis and endosomal recycling pathways.
  246. (1998). Phospholipase D is associated in a phorbol ester-dependent manner with protein kinase C-ʱ and with a 220-kDa protein which is phosphorylated on serine and threonine.
  247. (2009). Phospholipase D mechanism using Streptomyces PLD.
  248. (2000). Phospholipase D regulation and localisation is dependent upon a phosphatidylinositol 4, 5-bisphosphate-specific PH domain.
  249. (1997). Phospholipase D stimulates release of nascent secretory vesicles from the trans-Golgi network.
  250. (1999). Phospholipase D stimulation by receptor tyrosine kinases mediated by protein kinase C and a Ras/Ral signaling cascade.
  251. (1999). Phospholipase D structure and regulation.
  252. (2000). Phospholipase D1 is phosphorylated and activated by protein kinase C in caveolin-enriched microdomains within the plasma membrane.
  253. (1998). Phospholipase D1 localises to secretory granules and lysosomes and is plasma-membrane translocated on cellular stimulation.
  254. (2006). Phospholipase D1 regulates cell migration in a lipase activity-independent manner.
  255. (2001). Phospholipase D1b and D2a generate structurally identical phosphatidic acid species in mammalian cells.
  256. (2002). Phospholipase D2 is localized to the rims of the Golgi apparatus in mammalian cells.
  257. (2006). Phospholipase D2 is required for efficient endocytic recycling of transferrin receptors.
  258. (2004). Phospholipase D2 localizes to the plasma membrane and regulates angiotensin II receptor endocytosis.
  259. (2004). Phospholipase D2 modulates agonist-induced mu-opioid receptor desensitization and resensitization.
  260. (2007). Phospholipase D2-derived phosphatidic acid binds to and activates ribosomal p70 S6 kinase independently of mTOR.
  261. (2007). Phospholipase D2-generated phosphatidic acid couples EGFR stimulation to Ras activation by Sos.
  262. (1997). Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization.
  263. (2008). Phospholipid biosynthesis in eukaryotes.
  264. (2002). Phosphorylationdependent regulation of phospholipase D2 by protein kinase C delta in rat pheochromocytoma PC12 cells.
  265. (1998). PIPkins1, their substrates and their products: new functions for old enzymes.
  266. (2009). PKCε has an alcoholbinding site in its second cysteine-rich regulatory domain.
  267. (2007). Plasma lyso-phosphatidylcholine concentration is decreased in cancer patients with weight loss and activated inflammatory status. Lipids Health Dis.
  268. (2003). PLD1 Regulated mTOR signaling and mediates Cdc42 activation of S6K1.
  269. (1998). PLD2 complexes with the EGF receptor and undergoes tyrosine phosphorylation at a single site upon agonist stimulation.
  270. (1995). Properties of Rab5 Nterminal domain dictate prenylation of C-terminal cysteines.
  271. (2003). Protein-lipid interplay in fusion and fission of biological membranes.
  272. (1999). PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization.
  273. (1994). Purification and characterization of phosphatidylcholine phospholipase D from pig lung.
  274. (2004). Quantitative fluorescence resonance energy transfer (FRET) measurement with acceptor photobleaching and spectral unmixing.
  275. (1993). Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase.
  276. (1998). Rab GTPases, directors of vesicle docking.
  277. (2008). Rab13 regulates membrane trafficking between TGN and recycling endosomes in polarized epithelial cells.
  278. (1996). Raf-1 kinase possesses distinct binding domains for phosphatidylserine and phosphatidic acid. Phosphatidic acid regulates the translocation of Raf-1 in 12-Otetradecanoylphorbol-13-acetate-stimulated Madin-Darby canine kidney cells.
  279. (2004). Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis.
  280. (2000). Rapid report: inhibition of phosphatidylinositol 4,5-bisphosphate-stimulated phospholipase D2 activity by Ser/Thr phosphorylation.
  281. (2002). Rapidly maturing variants of Discosoma red fluorescent protein (DsRed).
  282. (2003). Ras superfamily monomeric G proteins in carcinoma cell motility.
  283. (2000). Rat basophilic leukaemia cells express syntaxin-3 and VAMP-7 in granule membranes.
  284. (2005). Recognition of phospholipids in Streptomyces phospholipase
  285. (1999). Recognizing the pleckstrin homology domain fold in mammalian phospholipase D using hidden Markov models.
  286. (2000). Regulation of human PLD1 and PLD2 by calcium and protein kinase
  287. (1998). Regulation of PDE-4 cAMP phosphodiesterases by phosphatidic acid.
  288. (1996). Regulation of phospholipase D by protein kinase C is synergistic with ADP-ribosylation factor and independent of protein kinase activity.
  289. (1995). Regulation of phospholipase D in HL60 cells. Evidence for a cytosolic phospholipase D.
  290. (2002). Regulation of phospholipase D.
  291. (2003). Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs.
  292. (2004). Regulation of phospholipase D2 by GTP-dependent interaction with dynamin.
  293. (1998). Regulation of phospholipase D2: selective inhibition of mammalian phospholipase D isoenzymes by alpha- and beta-synucleins.
  294. (1991). Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq.
  295. (2001). Requirements and effects of palmitoylation of rat PLD1.
  296. (2000). Review: Phospholipase D: molecular and cell biology of a novel gene family.
  297. Review: The roles of phospholipase D in EGFR signalling.
  298. (2004). Rho GTPases have diverse effects on the organization of the actin filament system.
  299. (2005). Rho GTPases: biochemistry and biology.
  300. (2001). Role for phospholipase D in receptormediated endocytosis.
  301. (2000). Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells.
  302. (1997). Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization.
  303. (2007). Role of EGF-induced tyrosine phosphorylation of reggie-1/flotillin-2 in cell spreading and signalling to the actin cytoskeleton.
  304. (2003). Secretory granule exocytosis.
  305. (1996). Secretory lysosomes – a special mechanism of regulated secretion in haemopoietic cells.
  306. (2002). Secretory lysosomes.
  307. (1999). Selective activation of phospholipase D2 by unsaturated fatty acid.
  308. (1997). Selective activation of rolipramsensitive, cAMP-specific phosphodiesterase isoforms by phosphatidic acid.
  309. (1997). Selective stimulation of a cAMP-specific phosphodiesterase (PDE4A5) isoform by phosphatidic acid molecular species endogenously formed in rat thymocytes.
  310. (2007). Sensor of phospholipids in Streptomyces phospholipase D.
  311. (1999). Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.
  312. Serine/threonine protein kinases synergistically regulate phospholipase D1 and 2 and secretion in RBL-2H3 mast cells.
  313. (2002). Short-term regulation of PDE4 activity.
  314. (1997). Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains.
  315. (2001). Signalling role for ARF and phospholipase D in mast cell exocytosis stimulated by crosslinking of the high affinity FcεR1 receptor.
  316. (2005). Sites of phospholipase D2 phosphorylated by PKCʱ.
  317. (1998). SMART, a simple modular architecture research tool: Identification of signaling domains.
  318. (1973). Solubilization and properties of a membranebound enzyme from rat brain catalyzing a base-exchange reaction.
  319. (2000). Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: Functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment.
  320. (2002). Specificity of Rho insert-mediated activation of phospholipase D1.
  321. (1996). Stimulation of actin stress fibre formation mediated by activation of phospholipase D.
  322. (1999). Structural analysis of human phospholipase
  323. (1998). Structural basis for activation of ARF GTPase: Mechanisms of guanine nucleotide exchange and GTP–Myristoyl Switching.
  324. (2003). Structural basis of the Rho GTPase signaling.
  325. (2007). Structural evidence for a common intermediate in small G protein-GEF reactions.
  326. (2005). Studies of the roles of ADP-ribosylation factors and phospholipase D in phorbol ester-induced membrane ruffling.
  327. (1997). Synaptojanin inhibition of phospholipase D activity by hydrolysis of phosphatidylinositol 4,5-bisphosphate.
  328. (1999). Synaptotagmin II negatively regulates Ca 2+-triggered exocytosis of lysosomes in mast cells.
  329. (2002). Synaptotagmin: a Ca 2+ sensor that triggers exocytosis?.
  330. (2007). Synaptotagmins I and IX function redundantly in regulated exocytosis but not endocytosis in PC12 cells.
  331. (2006). Synthesis and evaluation of fluorogenic substrates for phospholipase D and phospholipase
  332. (2002). TAPAS-1, a novel microdomain within the unique N-terminal region of the PDE4A1 cAMP-specific phosphodiesterase that allows rapid, Ca 2+-triggered membrane association with selectivity for interaction with phosphatidic acid.
  333. (2004). Targeted deletion of hepatic CTP:phosphocholine cytidylyltransferase alpha in mice decreases plasma high density and very low density lipoproteins.
  334. (2000). The cAMP-specific phosphodiesterase PDE4D3 is regulated by phosphatidic acid binding. Consequences for cAMP signaling pathway and characterization of a phosphatidic acid binding site.
  335. (1994). The cysteine-rich region of raf-1 kinase contains zinc, translocates to liposomes, and is adjacent to a segment that binds GTP-ras.
  336. (2000). The first crystal structure of a phospholipase D.
  337. (2003). The guanine nucleotide exchange factor ARNO mediates the activation of ARF and phospholipase D by insulin.
  338. (1988). The HL60 cell line: a model system for studying human myeloid cell differentiation.
  339. (2009). The influence of linoleic and linolenic acid on the activity and intracellular localisation of phospholipase D in COS-1 cells.
  340. (1981). The kinetics of the phospholipase A2-catalyzed hydrolysis of egg phosphatidylcholine in unilamellar vesicles. Product inhibition and its relief by serum albumin.
  341. (2000). The lysosomal protease cathepsin D is efficiently sorted to and secreted from regulated secretory compartments in the rat basophilic/mast cell line RBL.
  342. (2005). The mechanism of membrane targeting of human sphingosine kinase 1.
  343. (2006). The phox (PX) domain proteins and membrane traffic.
  344. (2001). The phox homology (PX) domain, a new player in phosphoinositide signalling.
  345. (2006). The phox homology domain of phospholipase D activates dynamin GTPase activity and accelerates EGFR endocytosis.
  346. (2005). The rate-limiting enzyme in phosphatidylcholine synthesis regulates proliferation of the nucleoplasmic reticulum.
  347. (2000). The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras.
  348. (2001). The role and relevance of phospholipase D1 during growth and dimorphism of Candida albicans.
  349. (1997). The role of ADPribosylation factor and phospholipase D in adaptor recruitment.
  350. (2002). The role of phosphatidic acid in the regulation of the Ras/MEK/Erk signaling cascade.
  351. (2003). The simultaneous production of phosphatidic acid and diacylglycerol is essential for the translocation of protein kinase Cepsilon to the plasma membrane in RBL-2H3 cells.
  352. (2008). The small GTP-binding protein rat regulates growth factor-induced membrane ruffling.
  353. (1996). Tissuespecific distribution and subcellular distribution of phospholipase D in rat: evidence for distinct RhoA- and ADP-ribosylation factor (ARF)-regulated isoenzymes.
  354. (2001). Trans-Golgi network sorting.
  355. (2008). Transglutaminasecatalyzed transamidation: A novel mechanism for Rac1 activation by 5-hydroxytryptamine2A receptor stimulation.
  356. (2003). Transmodulation between phospholipase D and c-Src enhances cell proliferation.
  357. (1967). Transphosphatidylation by phospholiase D.
  358. (2000). Type Ialpha phosphatidylinositol 4-phosphate 5-kinase is a putative target for increased intracellular phosphatidic acid.
  359. (2003). Understanding tubulin-Taxol interactions: mutations that impart Taxol binding to yeast tubulin.
  360. (1979). Unsaturated diacylclycerol as a possible messenger for the activation of calciumactivated, phospholipid-dependent protein kinase system.
  361. (1993). Upregulation of phospholipase D activity induced by over-expression of protein kinase 295 C-alpha. Studies in intact Swiss/3T3 cells and in detergent-solubilized membranes in vitro.
  362. (2004). Upstream and downstream of mTOR.
  363. (1995). Wortmannin and its structural analogue demethoxyviridin inhibit stimulated phospholipase A2 activity in Swiss 3T3 Cells. Wortmannin is not a specific inhibitor of phosphtidylinositol 3-kinase.

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