Location of Repository

Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus

By Tingting Cui, Jun Xiong, Yongzhi Wang, Xintian Wen, Xiaobo Huang, Yong Huang, Xiaoping Ma, Zhongkai Hu, Qin Zhao and Sanjie Cao

Abstract

The attenuated <em>Salmonella typhimurium χ</em>4550 strain was used to harbour a reconstructed bicistronic DNA vaccine against porcine rotavirus, which carried the rotavirus nonstructural protein 4 (<em>NSP4</em>) and VP7 genes simultaneously. Using a balanced lethal system, the kanamycin resistance gene of expressing eukaryotic plasmids pVAX1 and pVAXD were replaced by the aspartate β-semialdehyde dehydrogenase (<em>asd</em>) gene. The NSP4 cleavage product (259–525) of rotavirus OSU strain and VP7 full-length genes were amplified by reverse transcription polymerase chain reaction and then inserted into the eukaryotic single-expression plasmid, pVAX1-asd, and the eukaryotic dual-expression plasmid, pVAXD-asd, respectively. The recombinant plasmids pVAX1-asd-NSP4, pVAX1-asd-VP7 and pVAXD-asd-NSP4-VP7 were transformed into the attenuated <em>S. typhimurium χ</em>4550 strain by electrotransformation. An indirect immunofluorescence assay of the expressed COS-7 cell suggested that the recombinant <em>S. typhimurium χ</em>4550 strain was constructed successfully. The recombinant <em>S. typhimurium χ</em>4550 strain was orally administered to BALB/c mice. The group immunised with dual- expression plasmids produced a significantly higher level of serum Immunoglobulin G (IgG) and intestinal Immunoglobulin A (IgA) than the group immunised with single-expression plasmids. These results indicated that eukaryotic bicistronic plasmid DNA vaccines could be successfully constructed to enhance humoural, mucosal and cellular immune response against rotavirus infection

Topics: DNA vaccine, immune response, NSP4 gene, porcine rotavirus, Salmonella typhimurium χ4550 strain, VP7 gene, Veterinary medicine, SF600-1100
Publisher: AOSIS
Year: 2013
DOI identifier: 10.4102/ojvr.v80i1.498
OAI identifier: oai:doaj.org/article:b91be4b97fc74d4c900370af7e0fe1d0
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/2219-0635 (external link)
  • https://doaj.org/toc/0030-2465 (external link)
  • http://www.ojvr.org/index.php/... (external link)
  • https://doaj.org/article/b91be... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.