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INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS

By E. A. Starikova, I. S. Freidlin, D. I. Sokolov, S. A. Selkov, K. A. Sisoev and Areg A. Totolian

Abstract

<p><strong>Abstract. </strong>We demonstrated that TNFα, IFNγ and IL-4 induce different patterns of endothelial cells activation. The greatest alterations were observed in TNFα threated endothelial cells. Under the influence of this cytokine there was a statistically significant elevation of IL-8 and MCP-1 production by endothelial cells, of THP-1 adhesion and of migration toward endothelial cells monolayer. At the same time we had not observed any immunophenotype alteration of THP-1 cocultured with endothelial cells treated with TNFα.</p><p>IFNγ и IL-4 induced statistically significant inhibition of THP-1 adhesion. IL-4 treated endothelial cells have elevated levels of IL-8 and MCP-1 production and stimulated THP-1 migrations. IFNγ showed inhibition of IL-8 and MCP-1 spontaneous production and THP-1migration.</p><p>Incubation of THP-1 with untreated and IFNγ treated endothelial cells led to significant elevation of HLA-DR expression. Expression of CD68 was significantly higher in case of THP-1 cocultured with IL-4 treated endothelial cells. There was no any correlation between immunophenotype alteration THP-1cells and others parameters alteration.</p

Topics: эндотелиальные клетки, цитокины, моноциты, адгезия, миграция, фенотип, Immunologic diseases. Allergy, RC581-607
Publisher: SPb RAACI
Year: 2005
OAI identifier: oai:doaj.org/article:f8567dde2b1c4fcfaa031138a9c3d1dd
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