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Identification of the gene Rht8 with using of GWM 261 marker in forms of common wheat with diverse origins

By Agnieszka Tomkowiak, Magdalena Manikowska, Danuta Kurasiak-Popowska, Dorota Weigt, Jerzy Nawracała, Sylwia Mikołajczyk and Janetta Niemann


Lodging is the most important of the various factors affecting yielding in common wheat (Triticum aestivum L. ssp. vulgare). In breeding one of the most effective methods to obtain cultivars resistant to lodging is to introduce semi-dwarf genes. One of the genes used in wheat breeding programs worldwide is the Rht8 gene, whose presence is correlated with a reduction of stalk length by approximately 10%. This gene is sensitive to the action of exogenous gibberellic acid, thus it may be identified using microsatellite single sequence repeat (SSR) markers. The aim of this study was to identify the Rht8 gene using the GWM 261 marker in 15 winter wheat cultivars of various pedigree. The analyses showed the presence of specific products of 175 bp in cv. ‘Augusta’ and 165 bp in cvs. ‘Brevor’, ‘Greer’, ‘Augusta’, ‘Geneva’, ‘Karl’, ‘Pionier 2548’, ‘Pionier 2737W’. This cultivar may be used in breeding programs as a source of the Rht8 gene

Topics: wheat, semi-dwarf genes, Rht8, SSR markers, Agriculture (General), S1-972, Agriculture, S
Publisher: Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu
Year: 2016
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