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Expression and activation of mitogen-activated protein kinases in matured porcine oocytes under thermal stress

By Shih-Ying Yen, Jung-Kai Tseng, Show-Mei Chuang, Shuen-Ei Chen and Jyh-Cherng Ju

Abstract

In this study, we determined the expression and activation of p38 MAPK in matured porcine oocytes subjected to heat shock (HS). When MII oocytes were heated, only the phosphorylated p38 levels relative to the total p38 levels decreased (P < 0.01) after HS, but no clear relationship with HS treatments was observed in the ERK, JNK and p90(rsk) expressions of matured oocytes. To confirm p38 activation in matured oocytes, immunocytochemical staining was performed to localize its expression and distribution in the ooplasm, and the results were largely consistent with previous Western blot analyses. Moreover, when matured oocytes were co-cultured with a P38 MAPK inhibitor, SB203580, for 4 h at 41.5 C, the activation of its immediate downstream substrate MAPKAPK-2 was not inhibited within any of the treatment groups. It appears that the MAPKAPK2 levels increased only under prolonged culture (HS4h and C4h) compared with the control group. In conclusion, p38 activity in porcine oocytes was decreased after exposure to HS and prolonged culture. These alterations of p38 and activation of MAPKAPK2 may be associated with porcine oocyte viability under HS conditions, and a potential cross-talk between p38 MAPK and other signaling cascades may exist, which warrants additional investigation

Topics: Animals, Cells, Cultured, Cytoplasm, Enzyme Activation, Female, Hot Temperature, Immunohistochemistry, Oocytes, Phosphorylation, Time Factors, p38 Mitogen-Activated Protein Kinases, Heat-Shock Response, In Vitro Oocyte Maturation Techniques, Sus scrofa
Year: 2015
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/86560
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