利用RAPD與 ISSR分子標誌評估印度棗品種間之遺傳歧異度

Abstract

本研究已28個RAPD引子及16個ISSR引子增殖產生之標誌分析15個印度棗品種之遺傳歧異度。結果共獲得286個RAPD標誌片段及150個ISSR DNA片段。以RAPD全部增殖DNA片段分析所得相似度矩陣經UPGMA法建構之親緣樹狀圖顯示相似度介於0.68~0.95。以RAPD多型性標誌分析所得相似度矩陣經UPGMA法建構之親緣樹狀圖顯示相似度介於0.42~0.92。以ISSR全部增殖DNA片段分析所得相似度矩陣經UPGMA建構之親緣樹狀圖顯示相似度介於0.80~0.97。以ISSR多型性標誌分析所得相似度矩陣經UPGMA法建構之親緣樹狀圖顯示相似度介於0.58~0.91。The genetic diversity among 15 Indian jujube (Zizyphus mauritiana Lam.) cultivars were analyzed by RAPD and ISSR markers, generated by 28 RAPD primers and 16 ISSR primers. A total of 286 markers were scored by using the 16 ISSR primers. UPGMA analysis was performed and dendrograms were constructed. The similarity coefficients based on RAPD total amplified DNA fragments were between 0.68 and 0.95. The similarity coefficients based on RAPD polymorphic markers were between 0.42 and 0.92. The similarity coefficients based on ISSR total amplified DNA fragments were between 0.80 and 0.97. The similarity coefficients based on ISSR polymorphic markers were between 0.58 and 0.91