印度棗莖頂組織培養之研究

Abstract

本研究採用台灣栽培印度棗(Zizyphus mauritiana Lam.)主要品種高朗1號為試驗材料，探討培養基組成及培養方法對莖頂培養之影響。在新稍長旺盛期，採取長約5㎝頂部，切取長約0.5mm大小帶4個葉原體的生長點，植入1/2MS添加0.1 mg/l BA、0.5mg/l IBA之固態培養基進行初代培養，經30日後移植於相同配方之固態培養基，可以促進培植體生長。培植體經初代培養後移致增殖培養基中培養，以1/2MS添加0.5 mg/l kinetin及0.05 mg/l IBA之培養基培養枝捎30日後可獲得增值之枝梢。經增殖培養所得長10mm之莖頂扦插於含IBA 2.5 mg/l之1/2MS培養基中暗處理培養7日後，移至 auxin-free之培養基培養23日，可獲發根小苗。Shoot tip culture of the Kaung Laang No. 1 Indian Jujube (Zizyphus mauritiana Lam.) was studied, optimal micropropagation, shoot rooting and acclimatization were investigated. Shoot tip were harvested about 5 cm in length from actively field grown trees and shoot apices were excised 0.5mm for initial culture. The best grown were achieved when shoot apices culture on solid medium with 1/2MS strength of Murashige and Skoog (MS) containing BA 0.1 mg/l and IBA 0.5 mg/l, sucrose 30 g/l and agar 8 g/l and incubated in darkness for 1 days, then incubated in lightness for 29 days. These explants were transferred to the same component solid medium for futher growth. Multiple shoots were induced by 1/2MS medium containing sucrose 30 g/l and agar 8 g/l plus kinetin 0.05 mg/l and IBA 0.05mg/l for 30 days. For rooting, the shoots grew above 10 mm in height, were placed on 1/2MS medium IBA 2.5 mg/l, sucrose 30 g/l and agar 6 g/l and incubated in darkness for 7 days, then transplanted to an auxin-free 1/2MS medium for 23 days