Skip to main content
Article thumbnail
Location of Repository

Expression of NeuroD4 in brain tumor

By 歐玟蘭 and Wen-Lan Ou

Abstract

台灣原發性腦瘤的確診數一年約為一千八百至二千例,其中約有一千四百例死亡。由於腫瘤位處於腦部,密閉且構造複雜的腦室,使臨床治療難度較其他癌症來的高,除了手術切除腫瘤外,放射治療、化學治療效果皆有限。在本研究中,我們探討與腦部細胞分化相關的轉錄因子(transcription factor) NeuroD4( neurogenic differentiation 4) 在神經膠質母細胞瘤(glioblastoma multiforme,GBM)的表現,為了探究兩者之相關性,我們使用免疫組織化學染色法(immunohistochemistry;IHC)分析68個患者腦瘤病理組織切片。結果顯示NeuroD4在GBM的表現,整體陽性率為82.35%,且在越惡性的臨床病例中,表現量越高。的確證明NeuroD4的表現與腦瘤惡性程度有極大關聯性。未來將進一步探討其轉錄因子作用機制,以期將來成為在臨床診斷、預後及治療的新契機。There are about 1,800~2,000 cases of primary brain tumor per year in Taiwan. In those, about 1,400 persons die for the tumor. Because of the complicated ventricle and hard skull, it is more difficult to be cure than other cansers in clinical treatment. There is no effective way to cure brain tumors besides surgery. In this study, we investigate a transcription factor NeuroD4( neurogenic differentiation 4),which is related to brain cell differentiation, in GBM. We used immunohistochemistry to analyse 68 cases of brain tumor paraffin-embedded sections. Our results showed that the positive rate of NeuroD4 expression in GBM is 82.35%. In addition, its expression level was higher in the more malignant clinical cases. In this study, we demonstrate that the expression of NeuroD4 is linked to the malignant levels of brain tumor, and suggest that NeuroD4 can be a potential marker for clinical diagnosis, and prognosis.摘要........................................................I Summary....................................................II目錄......................................................III 圖表目錄...................................................IV 附錄及附錄圖目錄............................................V 第一章 緒論.................................................1 第一節 腦瘤概述.............................................1 第二節 NeuroD4(neurogenic differentiation 4)蛋白............5 第三節 腦瘤分子診斷.........................................7 第二章 實驗設計與研究目的..................................10 第三章 實驗材料及方法......................................11 第一節 實驗材料............................................11 一 腦瘤組織檢體............................................11 二 抗體(如附錄一)..........................................11 三 藥品試劑( 如附錄一).....................................11 四 緩衝溶液(如附錄一)......................................11 第二節 實驗方法............................................11 第四章 實驗結果............................................15 第一節 NeuroD4在腦瘤組織切片檢體中的表現...................15 第二節 NeuroD4表現量與腦瘤患者存活率分析...................15 第五章 討論................................................17 第六章 結論................................................21 參考文獻...................................................22 圖表.......................................................26 附錄及附錄圖...............................................33 圖表目錄 圖一 NeuroD4在GBM檢體中之表現,陰性結果顯微鏡下所見........26 圖二 NeuroD4在GBM檢體中之表現,1+陽性結果顯微鏡下所見......27 圖三 NeuroD4在GBM檢體中之表現,2+陽性結果顯微鏡下所見......28 圖四 由實驗數據與臨床診斷分析所得之存活曲線圖..............29 圖五 NeuroD protein family組成示意圖.......................30 表一 樣本免疫組織化學染色判讀表............................31 表二 良性及惡性腦瘤比較表..................................32 附錄及附錄圖目錄 附錄圖一 腦部構造圖........................................33 附錄圖二 神經元結構........................................33 附錄圖三 basic Helix-Loop-Helix domain結構圖...............34 附錄圖四 GBM病理切片特徵(HE stain) ........................35 附錄圖五 衛生署公布民國101年國民主要死因死亡率圖...........36 附錄圖六 衛生署公布以民國90年和101年國人十大死因比較分析圖.37 附錄一 抗體、藥品及緩衝液..................................3

Topics: 腦瘤, 神經膠質母細胞瘤, 轉錄因子, NeuroD4, neurogenic differentiation 4, brain tumor, glioblastoma multiforme, transcription factor
Publisher: 生命科學院碩士在職專班
Year: 2014
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/81146
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.airitilibrary.com/P... (external link)
  • http://hdl.handle.net/11455/81... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.