Establishment of multiplication protocols on medicinal plants Flemingia macrophylla (Willd.) Kuntze ex Prain and Macleaya cordata ( Willd. ) R. Br.

Abstract

本試驗目的在於探討藥用植物大葉佛來明豆及博落迴的最佳培養條件，建立有效的增殖系統。研究方法使用組織培養技術，誘導培植體長芽與發根。大葉佛來明豆芽體增殖試驗以生長調節劑和節位兩因子處理，結果顯示以添加BA 0.2 mg L-1和NAA 0.02 mg L-1之B5培養基培養八週可誘導出較多的芽，為6.6個，但是有葉片縮小的變異；無菌苗的四個節位中，第1節位芽體增殖效果最佳，可得6.3個芽。發根試驗結果為B5培養基中添加NAA 0.1 mg L-1發根率可達100%，且根部較粗且長。博落迴小芽以MS培養基添加BA 0.05 mg L-1、NAA 0.005 mg L-1進行芽體增殖，六週後平均可獲得4.8個芽，並且有產生叢生芽；而誘導葉柄產生不定芽時，B5添加BA 0.1 mg L-1培養八週的不定芽誘導率最高，為47.22%，且芽體變異情況較低，比MS培養基更適合誘導不定芽。The aim of this study were researching the best culture condition of medicinal plants of Flemingia macrophylla (Willd.) Kuntze ex Prain and Macleaya cordata ( Willd. ) R. Br. with the method of tissue culture. The results indicated that in vitro micropropagation of F. macrophylla (Willd.) Kuntze ex Prain produced maximum shoots (6.6) by cultivating on B5 medium supplemented with BA 0.2mg L-1 and NAA 0.02 mg L-1, but there was mutant shrinking leaves. In four bud positions of the sterile seedlings, the first bud position growing 6.3 shoots was the best shoot proliferation choice. In rooting experiment, using B5 medium supplemented with NAA 0.1 mg L-1 could get good rooting rate as 100%. Shoots of M. cordata ( Willd. ) R. Br. could proliferate more buds (4.8) in MS medium supplemented with BA 0.05 mg L-1 and NAA 0.005 mg L-1 than other treatments. B5 adding BA 0.1 mg L-1 could get the highest inducing rate (47.22%) when inducing adventitious buds from petioles of M. cordata ( Willd. ) R. Br. and had less mutant buds, so was better than MS for induction of adventitious buds.摘 要 i Abstract ii 目 次 iii 表目次 iv 圖目次 v 縮寫表 vi 前 言 1 前人研究 2 材料與方法 7 結 果 9 討 論 27 結 論 33 參考文獻 34 附 錄 4