Ceske Budejovice (Czech Republic) : Jihoceska univerzita v Ceskych Budejovicich
Abstract
This thesis consists of three published articles and one submitted manuscript, where there is contributed the finding that a new coactivator MBF1 interacts in transferred cultured cells with a Drosophilla ortholog of the oncoprotein Jun. The first of the published articles describes the heat-inducible transgenic expression in the Bombyx mori silkmoth. The other two publications explore the use of recombinant Sindbis viral vectors for the protein expression and RNAi gene knock-downs in the silkmoth. A Sindbis-mediated knock-down has been demonstrated for an ecdysone-dependent Broad-Complex transcription factor, whose central role in metamorphosis has thus been confirmed in the first non-drosophillid species.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi
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