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Fractionation on Cross-Linked Dextran, Sephadex G-25, of Sera from Sheep Infected with Johne's Bacilli. Preliminary Report

By K. F. Mori


Ten sera collected during the winter months from six sheep infected with Johne's bacilli were fractionated on Sephadex G-25 columns, and all fractions tested for complement-fixing antibody, anticomplementary properties and for supplementing and inhibitory activities when added to complement-fixation tests of a heterologous antigen-antibody system: bovine or ovine antiserum with Brucella abortus antigen. Serum from a normal sheep was similarly fractionated and examined

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