Skip to main content
Article thumbnail
Location of Repository

N-glycans of recombinant human acid α-glucosidase expressed in the milk of transgenic rabbits

By S.P. Jongen, G.J. Gerwig, B.R. Leeflang, K. Koles, M.L.M Mannesse, P.H.C. van Berkel, F.R. Pieper, M.A. Kroos, A.J.J. Reuser, Q. Zhou, X. Jin, K. Zhang, T. Edmunds and J.P. Kamerling

Abstract

Pompe disease is a lysosomal glycogen storage disorder characterized by acid α-glucosidase (GAA) deficiency. More than 110 different pathogenic mutations in the gene encoding GAA have been observed. Patients with this disease are being treated by intravenous injection of recombinant forms of the enzyme. Focusing on recombinant approaches to produce the enzyme means that specific attention has to be paid to the generated glycosylation patterns. Here, human GAA was expressed in the mammary gland of transgenic rabbits. The N-linked glycans of recombinant human GAA (rhAGLU), isolated from the rabbit milk, were released by peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase F. The N-glycan pool was fractionated and purified into individual components by a combination of anion-exchange, normal-phase, and Sambucus nigra agglutinin-affinity chromatography. The structures of the components were analyzed by 500 MHz one-dimensional and 600 MHz cryo two-dimensional (total correlation spectroscopy [TOCSY] nuclear Overhauser enhancement spectroscopy) 1H nuclear magnetic resonance spectroscopy, combined with two-dimensional 31P-filtered 1H-1H TOCSY spectroscopy, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and high-performance liquid chromatography (HPLC)-profiling of 2-aminobenzamide-labeled glycans combined with exoglycosidase digestions. The recombinant rabbit glycoprotein contained a broad array of different N-glycans, comprising oligomannose-, hybrid-, and complex-type structures. Part of the oligomannose-type glycans showed the presence of phospho-diester-bridged N-acetylglucosamine. For the complex-type glycans (partially) (α2-6)-sialylated (nearly only N-acetylneuraminic acid) diantennary structures were found; part of the structures were (α1-6)-core-fucosylated or (α1-3)-fucosylated in the upper antenna (Lewis x). Using HPLC-mass spectrometry of glycopeptides, information was generated with respect to the site-specific location of the various glycan

Year: 2007
OAI identifier: oai:dspace.library.uu.nl:1874/27471
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://dspace.library.uu.nl:80... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.