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Isoelectric points of proteins, determined by isoelectric focusing in the presence of urea and ethanol

By W.J. Gelsema, C.L. de Ligny and N.G. van der Veen


Isoelectric points, pIapp, in sucrose-urea-water and glycerol-ethanol-water mixtures and isoelectric points, pI, in water have been determined at 25° for some carrier ampholytes. The differences, pIapp---pI, are shown to account for the primary medium effect and the pH measuring cell effect on the isoelectric point. The diferences, pIapp---pI, for Ampholines are used to correct apparent isoelectric points of proteins. pI shifts resulting from the denaturing effect of urea and ethanol are discussed in terms of the conformation change

Topics: Scheikunde
Year: 1979
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