Isoelectric points, pIapp, in sucrose-urea-water and glycerol-ethanol-water mixtures and isoelectric points, pI, in water have been determined at 25° for some carrier ampholytes. The differences, pIapp---pI, are shown to account for the primary medium effect and the pH measuring cell effect on the isoelectric point. The diferences, pIapp---pI, for Ampholines are used to correct apparent isoelectric points of proteins. pI shifts resulting from the denaturing effect of urea and ethanol are discussed in terms of the conformation change
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