Diphosphatidylglycerol (cardiolipin) biosynthesis from CDP-diglyceride and\ud sn-glycerol-3-phosphate has been demonstrated in a preparation of mitochondria from\ud rat liver in a reaction requiring Mg²⁺. The identity of the diphosphatidylglycerol was\ud established by thin-layer chromatography and mild alkaline hydrolysis. Diphosphatidylglycerol\ud formation from [³H]phosphatidylglycerol was shown to occur only in the\ud presence of CDP-diglyceride. The rate of diphosphatidylglycerol formation appears\ud to be quite slow in mitochondria and may explain why it was not previously observed.\ud Evidence is presented which eliminates the possibility of a bacterial contribution to\ud the biosynthetic process observed
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