Cyclic 3′,5′-AMP phosphodiesterase of Saccharomyces carlsbergensis Inhibition by adenosine 5′-triphosphate, inorganic pyrophosphate and inorganic polyphosphate

Abstract

1. 1. Cyclic 3′,5′-AMP phosphodiesterase activity can be demonstrated in Saccharomyces carlsbergensis by measuring the 5′-AMP formation from cyclic 3′,5′-AMP. 2. 2. Enzymic activity was optimal at pH 8.5 and shows a 2-fold stimulation in the presence of 4 mM Mn2+; Mg2+, Ca2+ and EDTA hardly show any effect. 3. 3. ATP, inorganic polyphosphate and pyrophosphate are effective inhibitors of the phosphodiesterase. The nature of these inhibitions was found to be of the mixed type. 4. 4. The possible physiological significance of this inhibition is discussed