Skip to main content
Article thumbnail
Location of Repository

The synthesis and utilization of dCDP-diglyceride by a mitochondrial fraction from rat liver

By J. ter Schegget, H. van den Bosch, M.A. van Baak, K.Y. Hostetler and P. Borstq

Abstract

1. 1. Mitochondrial preparations from rat liver incorporate added dCTP into a compound that is readily extracted with chloroform. This compound was identified as dCDP-diglyceride by co-chromatography with authentic dCDP-diglyceride on silica gels with three different solvent systems and by the absence of an effect of borate on its RF value.\ud \ud 2. 2. The apparent Km for dCTP in dCDP-diglyceride synthesis by intact mitochondria was 4 · 10−5 M; the vmax was 1.1 · 10−2 nmoles/min per mg protein. Synthesis was inhibited by rCTP, whereas the incorporation of the rCDP moiety of rCTP into rCDP-diglyceride was inhibited by dCTP.\ud \ud 3. 3. dCDP-Diglyceride substituted for rCDP-diglyceride in the synthesis of phosphatidylglycerol from sn-glycero-3-phosphate by these mitochondrial preparations. The apparent Km's were 2 · 10−5 M for dCDP-diglyceride and 7 · 10−5 M for rCDP-diglyceride. The vmax was 0.42 · 10−2 nmole/min per mg protein with dCDP-diglyceride and 2.5 · 10−2 nmole/min per mg protein with rCDP-diglyceride as substrate

Topics: Scheikunde
Year: 1971
OAI identifier: oai:dspace.library.uu.nl:1874/24582
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://dspace.library.uu.nl:80... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.