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Electrophoresis simulated with the cage model for reptation

By A. van Heukelum and H.R.W. Beljaars

Abstract

The cage model for polymer reptation is extended to simulate gel electrophoresis. With increasing\ud electric field strength E, the drift velocity v of a long polymer with length L shows three different\ud regimes: (a) the linear regime where v~E/ L; (b) the quadratic regime where v~E^2 , independent\ud of the length of the polymer; and (c) a regime where the velocity decreases exponentionally with E.\ud The transition between regimes (a)! and (b) occurs for field strengths E~L^-1 . The transition\ud between regimes (b)! and (c) occurs for some value Eh , for which L^-1 <<Eh <<1. The behavior in the\ud first two regimes is in agreement with earlier reports on simulations of the Duke-Rubinstein model,\ud and with experimental work on DNA polymers in agarose gel. The third regime is not reported for\ud the Duke-Rubinstein model, probably because in this model, stored length cannot compile into\ud hernias

Topics: Natuur- en Sterrenkunde
Year: 2000
OAI identifier: oai:dspace.library.uu.nl:1874/1452

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