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Expression of Barley Endopeptidase B in Trichoderma reesei

By R. Saarelainen, A. Mantyla, H. Nevalainen and P. Suominen

Abstract

The gene for barley endopeptidase B (EPB) has been expressed in the filamentous fungus Trichoderma reesei from the cbh1 promoter. The EPB signal sequence allowed secretion of over 90% of the recombinant protein. Yields reached about 500 mg of immunoreactive protein per liter and exceeded values for any other protein derived from a higher eukaryotic organism produced in T. reesei

Topics: Research Article
OAI identifier: oai:pubmedcentral.nih.gov:1389312
Provided by: PubMed Central
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