Heat sterilization of archaeal liposomes

Abstract

Thermal stabilities were compared between liposomes prepared from the ether lipids extracted from various archaeobacteria and liposomes composed of ester lipids. Leakage of entrapped carboxyfluorescein from the liposomes exposed to 121 \ub0C indicated a marked stability of certain ether liposomes, comparable or superior to cholesterol-stabilized liposomes prepared from the saturated synthetic lipids dimyristoyl phosphatidylcholine and dimyristoyl phosphatidylglycerol. The heat stability of diether liposomes could be increased by the inclusion of tetraether lipids.Key words: archaeal liposomes, ether liposomes, heat sterilization, thermal stability, archaeobacteria ("Archaea").NRC publication: Ye