A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The
method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-
PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community
analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA
derived) down the horizons of an established grassland soil
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