Location of Repository

Recent Applications of DNA Microarray Technology to Toxicology and Ecotoxicology

By Teresa Lettieri

Abstract

Gene expression is a unique way of characterizing how cells and organisms adapt to changes in the external environment. The measurements of gene expression levels upon exposure to a chemical can be used both to provide information about the mechanism of action of the toxicant and to form a sort of “genetic signature” for the identification of toxic products. The development of high-quality, commercially available gene arrays has allowed this technology to become a standard tool in molecular toxicology. Several national and international initiatives have provided the proof-of-principle tests for the application of gene expression for the study of the toxicity of new and existing chemical compounds. In the last few years the field has progressed from evaluating the potential of the technology to illustrating the practical use of gene expression profiling in toxicology. The application of gene expression profiling to ecotoxicology is at an earlier stage, mainly because of the the many variables involved in analyzing the status of natural populations. Nevertheless, significant studies have been carried out on the response to environmental stressors both in model and in nonmodel organisms. It can be easily predicted that the development of stressor-specific signatures in gene expression profiling in ecotoxicology will have a major impact on the ecotoxicology field in the near future. International collaborations could play an important role in accelerating the application of genomic approaches in ecotoxicology

Topics: Commentaries & Reviews
Publisher: National Institute of Environmental Health Sciences
OAI identifier: oai:pubmedcentral.nih.gov:1332648
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Preview

    Citations

    1. (1999). Application of complementary DNA microarray technology to carcinogen identifi-cation, toxicology, and drug safety evaluation.
    2. (2003). ArrayExpress—a public repository for microarray gene expression data at the EBI.
    3. (2004). Clofibrate-induced gene expression changes in rat liver: a cross-laboratory analysis using membrane cDNA arrays. Environ Health Perspect 112:428–438.
    4. (2003). Genomic and proteomic profiling of responses to toxic metals in human lung cells. Environ Health Perspect 111:825–835.
    5. (2004). Identification of putative gene-based markers of renal toxicity. Environ Health Perspect 112:465–479.
    6. (2001). Minimum information about a microarray experiment (MIAME)—toward standards for microarray data.
    7. (2001). RNA expression in the early characterization of hepatotoxicants in Wistar rats by high-density DNA microarrays. Hepatology 33:1239–1258.
    8. (2004). Submission of microarray data to public repositories.
    9. (2004). The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism.
    10. (2001). Unique gene expression patterns in liver and kidney associated with exposure to chemical toxicants.
    11. (2004). Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides). Mar Environ Res 58:565–569.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.