Skip to main content
Article thumbnail
Location of Repository

Light-scattering studies of bull spermatozoa. II. Interaction and concentration effects.

By M W Woolford and J D Harvey


The complete autocorrelation function of the intensity fluctuations of laser light scattered from motile bull spermatozoa is shown to depend upon several factors not previously considered. Samples of bull spermatozoa generally contain a substantial proportion of dead cells, which give rise to slowly decaying components of the autocorrelation function. Whereas previous work has concentrated on the form of the fast decaying autocorrelation component, we are concerned here with the relative amplitude and shape of the slow autocorrelation component and the general form of the composite function. In principle, the relative amplitudes of the fast and slow components of the autocorrelation function can be used as an assay of the proportion of swimming cells. We show that this amplitude ratio depends upon cell concentration, scattering cell geometry, and scattering angle. A simple model is developed to explain these results on the basis of the asymmetry of light scattered from these cells, motile/immotile cell interactions, wall-swimming effects, and geotactic reorientation of dead cells

Topics: Research Article
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.