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Rapid and Specific Detection of the O15:K52:H1 Clonal Group of Escherichia coli by Gene-Specific PCR

By James R. Johnson, Krista Owens, Montse Sabate and Guillem Prats

Abstract

Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates

Topics: Bacteriology
Publisher: American Society for Microbiology
Year: 2004
DOI identifier: 10.1128/JCM.42.8.3841-3843.2004
OAI identifier: oai:pubmedcentral.nih.gov:497591
Provided by: PubMed Central
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