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Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a direct immunoperoxidase procedure.

By G Laurent, M F Gourdin and F Reyes

Abstract

Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution

Topics: Research Article
Year: 1982
DOI identifier: 10.1136/jcp.35.2.139
OAI identifier: oai:pubmedcentral.nih.gov:497486
Provided by: PubMed Central
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