Article thumbnail
Location of Repository

Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a direct immunoperoxidase procedure.

By G Laurent, M F Gourdin and F Reyes


Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution

Topics: Research Article
Year: 1982
DOI identifier: 10.1136/jcp.35.2.139
OAI identifier:
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.