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Use of monoclonal antibodies to recognise osteoclasts in routinely processed bone biopsy specimens.

By N A Athanasou, B Puddle, J Quinn and C G Woods


In decalcified (5% nitric acid) and undecalcified (glycol-methacrylate or resin embedded) routinely processed bone specimens osteoclasts against resorbing surfaces were identified with monoclonal antibodies directed against leucocyte common antigen (LCA) (PD7/26, 2B11), CD68 (KP1), and gpIIIa (Y2/51) but not against HLA-DR (CR3/43 and Ta11B5). Mononuclear cells on resorbing surfaces and occasional mononuclear cells against or near resting surfaces showed a similar pattern of reactivity. This study shows that immunohistochemistry is a sensitive and useful technique for identifying osteoclasts in routinely processed bone specimens. It also suggests a role for mononuclear cells (possibly pre-osteoclasts) in bone resorption

Topics: Research Article
Year: 1991
DOI identifier: 10.1136/jcp.44.8.664
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Provided by: PubMed Central
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