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Estimation of serum haemoglobin-binding capacity (haptoglobin) on Sephadex G.100

By A. P. Ratcliff and J. Hardwicke

Abstract

A method is described for the estimation of serum haptoglobin as `haemoglobin-binding capacity'. The method relies on column chromatographic separation of the haemoglobin/haptoglobin complex from excess added free haemoglobin on the dextran gel Sephadex G.100. The method is simple and reproducible, and correlates well with another method of estimating haemoglobin-binding capacity over a wide range of values

Topics: Articles
Year: 1964
DOI identifier: 10.1136/jcp.17.6.676
OAI identifier: oai:pubmedcentral.nih.gov:480856
Provided by: PubMed Central
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