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A Limited Number of Globin Genes in Human DNA

By Roberto Gambino, Daniel Kacian, Joyce O'Donnell, Francesco Ramirez, Paul A. Marks and Arthur Bank

Abstract

The number of globin genes in human cells was determined by hybridizing DNA from human spleens to (3)H-labeled DNA complementary to human globin mRNA. Assuming the rates of reannealing of complementary DNA and cellular DNA are similar, the extent of hybridization of complementary DNA at various ratios of cellular DNA to complementary DNA indicate that there are fewer than 10 globin gene copies per haploid human genome. An alternative analysis of the data, which introduces no assumptions concerning the relative rates of reaction of complementary DNA and cellular DNA, indicates fewer than 20 globin gene copies are present. DNA isolated from the spleen of a patient with β(+) thalassemia contained a number of globin gene copies similar to that of normal DNA

Topics: Biological Sciences: Biochemistry
Year: 1974
DOI identifier: 10.1073/pnas.71.10.3966
OAI identifier: oai:pubmedcentral.nih.gov:434307
Provided by: PubMed Central
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