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DNA synthesis in vitro dependent upon phiX174 replicative form I DNA.

By C Sumida-Yasumoto, A Yudelevich and J Hurwitz

Abstract

Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phiX RFII. The reaction was inhibited by nalidixic acid and novobiocin but not by rifampicin. DNA synthesis required the phiX174 gene A product and E. coli gene products dnaB, dnaC(D), dnaG, and rep

Topics: Research Article
Year: 1976
DOI identifier: 10.1073/pnas.73.6.1887
OAI identifier: oai:pubmedcentral.nih.gov:430412
Provided by: PubMed Central
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