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Copper Transport Across Pea Thylakoid Membranes

By Richard Shingles, Larry E. Wimmers and Richard E. McCarty


The initial rate of Cu(2+) movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu(2+)-sensitive fluorophore Phen Green SK. Cu(2+) transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu(2+) concentration and saturated at about 0.6 μm total Cu(2+). Cu(2+) uptake was maximal at a thylakoid lumen pH of 7.0. Cu(2+) transport was inhibited by Zn(2+) but was largely unaffected by Mn(2+) and Cu(+). Zn(2+) inhibited Cu(2+) transport to a maximum of 60%, indicating that there may be more than one transporter for copper in pea thylakoid membranes

Topics: Research Article
Publisher: American Society of Plant Biologists
Year: 2004
DOI identifier: 10.1104/pp.103.037895
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Provided by: PubMed Central
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