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Studies on In Vitro DNA Synthesis Isolation of dna B Gene Product from Escherichia coli

By Michel Wright, Sue Wickner and Jerard Hurwitz

Abstract

Formation of duplex DNA from ϕX174 single-stranded DNA by extracts of E. coli was previously shown to require the gene product of dna B. Using as an assay the stimulation of ϕX174 DNA-dependent synthesis in inactivated extracts of dna B temperature-sensitive cells, we purified the dna B gene product from wild-type E. coli as well as from a dna B temperature-sensitive mutant. The dna B temperature-sensitive gene product is more thermolabile than its wild-type counterpart

Topics: Biological Sciences: Biochemistry
Year: 1973
DOI identifier: 10.1073/pnas.70.11.3120
OAI identifier: oai:pubmedcentral.nih.gov:427183
Provided by: PubMed Central
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