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Relation of Detergent HLB Number to Solubilization and Stabilization of D-Alanine Carboxypeptidase from Bacillus subtilis Membranes

By Jay N. Umbreit and Jack L. Strominger


The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effective in solubilizing the enzyme were effective in stabilizing it

Topics: Biological Sciences: Biochemistry
Year: 1973
DOI identifier: 10.1073/pnas.70.10.2997
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Provided by: PubMed Central
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