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Absence of Detectable IgM in Enzymatically or Biosynthetically Labeled Thymus-Derived Lymphocytes

By B. Lisowska-Bernstein, A. Rinuy and P. Vassalli

Abstract

Surface proteins of mouse thymus and spleen cells were radioiodinated with lactoperoxidase. After solubilization, the labeled proteins were precipitated by antibodies directed against mouse immunoglobulin chains; the precipitates were analyzed by radioautography after Na dodecyl sulfate-gel electrophoresis. Radioactive μ and L chains were absent from thymocyte extracts and conspicuous in spleen-cell extracts. The following cells were biosynthetically labeled for 4 hr [(35)S]methionine or 24 hr with [(14)C]leucine: (1) Thymocytes, (2) cortisoneresistant thymocytes [both treated with rabbit antisera cytotoxic to bone marrow-derived (B) lymphocytes and IgM-containing plasma cells, to kill possible contaminating nonthymus-derived cells], (3) “activated thymocytes” (allogeneic cell cultures of cortisone-resistant thymocytes), (4) human Daudi cells (a B lymphoblastic cell line), and (5) purified mouse B spleen lymphocytes devoid of plasma cells. Again no μ and L chains could be detected in thymocyte or thymus-derived cell extracts by immune precipitation and gel electrophoresis, while these chains were conspicuous in B-cell extracts. “Educated thymocytes,” obtained from spleens of lethally irradiated mice injected with syngeneic thymocytes and antigen, synthesized μ and L chains under similar conditions; this synthesis resulted from contamination of these cells by IgM-containing plasma cells

Topics: Biological Sciences: Immunology
Year: 1973
DOI identifier: 10.1073/pnas.70.10.2879
OAI identifier: oai:pubmedcentral.nih.gov:427130
Provided by: PubMed Central
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