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Synthesis of Inducible Tyrosine Aminotransferase in a Cell-Free Extract from Cultured Hepatoma Cells

By Jean-Paul Beck, Giselle Beck, K. Y. Wong and Gordon M. Tomkins


A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical

Topics: Biological Sciences: Biochemistry
Year: 1972
DOI identifier: 10.1073/pnas.69.12.3615
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Provided by: PubMed Central
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