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Glycyl-tRNA Synthetase: An Oligomeric Protein Containing Dissimilar Subunits*

By Dennis L. Ostrem and Paul Berg

Abstract

Glycyl-tRNA synthetase from Escherichia coli is a tetramer containing two small (33,000 daltons) and two large (80,000 daltons) subunits. The subunits can be separated and then reassembled to form active enzyme. Genetically altered glycyl-tRNA synthetases are modified in either the large or the small subunit. Wild type activity can be reconstituted in vitro by mixing extracts containing enzymes with modifications in different subunits

Topics: Biological Sciences: Biochemistry
Year: 1970
OAI identifier: oai:pubmedcentral.nih.gov:283454
Provided by: PubMed Central
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