Location of Repository

Fluorescent Probes of Biological Membranes

By Alan S. Waggoner and Lubert Stryer

Abstract

Fluorescent and phosphorescent probes that have readily interpretable emission properties can be specifically inserted into biological macromolecules to reveal facets of their structure and dynamics: (1) Proximity. Singlet-singlet and triplet-singlet energy transfer can serve as spectroscopic rulers in the 10-65 Å range, whereas triplet-triplet transfer can be used to show that two groups are less than about 12 Å apart. (2) Rotational mobility. Nanosecond fluorescence polarization measurements can reveal whether a macromolecular system has any modes of flexibility in times of nanoseconds. (3) Polarity. The presence of mobile dipoles in the environment of certain chromophores is reflected in their fluorescence quantum yield and emission spectrum

Topics: National Academy of Sciences
Year: 1970
OAI identifier: oai:pubmedcentral.nih.gov:283246
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.