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Control of Nitrate Reductase Activity in Barley Aleurone Layers*

By T. E. Ferrari and J. E. Varner

Abstract

Nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers has been determined in the intact tissue, using two different methods. The first method measures the rate of appearance of H218O produced during the reduction of KN18O3. The second assay measures excreted nitrite resulting from nitrate reduction under anaerobic conditions. Nitrite production in this anaerobic, intact-tissue assay was dependent upon the presence of phosphate (pH 7.5) and was increased by ethanol and bisulfite

Topics: Biological Sciences: Biochemistry
Year: 1970
OAI identifier: oai:pubmedcentral.nih.gov:282967
Provided by: PubMed Central
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