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ENZYMATIC REDUCTION OF SELENITE

By Walter J. Nickerson and Giuseppe Falcone

Abstract

Nickerson, Walter J. (Rutgers, The State University, New Brunswick, N.J.) and Giuseppe Falcone. Enzymatic reduction of selenite. J. Bacteriol. 85:763–771.—1963. Extraction of boiled yeast extract with n-hexane removed its ability to restore selenite-reducing capacity in dialyzed enzyme preparations obtained from baker's yeast or from Candida albicans. Menadione or thiodione substituted for the quinone(s) extracted by n-hexane. Active components of the water-soluble, dialyzable fraction of yeast extract included: glucose-6-phosphate, triphosphopyridine nucleotide, and glutathione. Selenite appears to be bound to protein through vicinal thiol groups, and to be released therefrom as metallic selenium after accepting four electrons

Topics: Articles
Year: 1963
OAI identifier: oai:pubmedcentral.nih.gov:278223
Provided by: PubMed Central
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