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NEW MODE OF GENETIC TRANSFER IN STREPTOCCUS FAECALIS VAR. LIQUEFACIENS1

By Ruth E. Raycroft and L. N. Zimmerman

Abstract

Raycroft, Ruth E. (The Pennsylvania State University, University Park), and L. N. Zimmerman. New mode of genetic transfer in Streptococcus faecalis var. liquefaciens. J. Bacteriol. 87:799–801. 1964.—When chloramphenicol-resistant mutants are grown in mixed culture with the sensitive wild type of Streptococcus faecalis var. liquefaciens 31, a transfer of chloramphenicol resistance occurs. (The marker is stable through at least five transfers.) The transfer mechanism is not inhibited by deoxyribonuclease, nor is it phage-mediated; genetic transfer is apparently dependent upon cell contact. Although kinetic studies have yet to be completed, preliminary data indicate a high efficiency (2.2 per donor cell in some experiments) of transmission. Two levels of drug resistance, 7 and 50 μg, were transferred; in each case, recipient cells expressed the donor's degree of resistance

Topics: Articles
Year: 1964
OAI identifier: oai:pubmedcentral.nih.gov:277095
Provided by: PubMed Central
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