Skip to main content
Article thumbnail
Location of Repository

Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection.

By E Tamburrini, P Mencarini, A De Luca, A Antinori, E Visconti, A Ammassari, L Ortona, E Ortona, A Siracusano and G Vicari

Abstract

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection

Topics: Research Article
Year: 1993
OAI identifier: oai:pubmedcentral.nih.gov:266016
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.