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Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates.

By M A Pfaller, Q Vu, M Lancaster, A Espinel-Ingroff, A Fothergill, C Grant, M R McGinnis, L Pasarell, M G Rinaldi and L Steele-Moore


MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories

Topics: Research Article
Year: 1994
OAI identifier: oai:pubmedcentral.nih.gov:263742
Provided by: PubMed Central
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