Location of Repository

Separation of Two Functional Roles of l-Alanine in the Initiation of Bacillus subtilis Spore Germination

By Richard Wax, Ernst Freese and Michael Cashel

Abstract

Spores of the standard transformable Marburg strain of Bacillus subtilis can be initiated to germinate by l-alanine alone. We isolated mutants which required for this process, in addition to l-alanine, the combination of d-glucose + d-fructose + K+ or NH4+ ions. In place of fructose, autoclaved or caramelized glucose could be used. Even the standard type strain required the addition of these three agents when d-alanine was present or when the temperature was raised. These findings show that l-alanine normally performs two functions during initiation, one of which is absent in the mutants or is blocked by d-alanine or elevated temperature. One of our mutants was not absolutely dependent on the addition of external l-alanine, because it could be initiated at a reduced rate by the sole addition of glucose + K+ or NH4+. When K+ or NH4+ was replaced by Na+, the initiation rate was greatly reduced. The divalent metal ions Mg++, Mn++, and Ca++ could not satisfy the cation requirement

Topics: Microbial Physiology and Metabolism
Year: 1967
OAI identifier: oai:pubmedcentral.nih.gov:251917
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefor we are unable to provide a PDF.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.