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Reductive Pentose Cycle and Formate Assimilation in Rhodopseudomonas palustris

By Jean E. Stokes and Derek S. Hoare


Rhodopseudomonas palustris assimilated formate autotrophically as carbon dioxide and hydrogen arising from the activity of the formic hydrogenlyase system. Kinetic analyses of cell suspensions pulse-labeled with 14C-formate or 14C-bicarbonate showed similar distributions of incorporated radioactivity. In both cases phosphate esters were the first assimilation products. Ribulose diphosphate carboxylase, phosphoribose isomerase, and phosphoribulokinase, characteristic enzymes of the reductive pentose cycle, were present in extracts of cells grown on formate

Topics: Microbial Physiology and Metabolism
Year: 1969
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Provided by: PubMed Central
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