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T Cell Integrin Overexpression as a Model of Murine Autoimmunity

By Raymond L. Yung, Donna Ray, Ru Ran Mo and Jun Chen

Abstract

Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell adoptive transfer model to investigate the role of T cell integrin adhesion molecules in the development of autoimmunity. This report summarizes the methods we used in establishing this murine model. By treating murine CD4+ T cells with DNA hypomethylating agents and by transfection we were able to test the in vitro effects of integrin overexpression on T cell autoreactive proliferation, cytotoxicity, adhesion and trafficking. Furthermore, we showed that the ability to induce in vivo autoimmunity may be unique to the integrin lymphocyte function associated antigen-1 (LFA-1)

Topics: Research Article
Publisher: Biological Procedures Online
Year: 2003
DOI identifier: 10.1251/bpo64
OAI identifier: oai:pubmedcentral.nih.gov:248482
Provided by: PubMed Central

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