Aim To evaluate in a rat animal model whether ovariectomy,
high fat diet (HFD), and physical activity in the form
of running affect leptin receptor (Ob-R) distribution in the
brain and white fat tissue compared to sham (Sh) surgery,
standard diet (StD), and sedentary conditions.
Methods The study included 48 female laboratory Wistar
rats (4 weeks old). Following eight weeks of feeding with
standard or HFD, rats were subjected to either OVX or Sh
surgery. After surgery, all animals continued StD or HFD
for the next 10 weeks. During these 10 weeks, ovariectomy
and Sh groups were subjected to physical activity or
sedentary conditions. Free-floating immunohistochemistry
and Western blot methods were carried out to detect
Ob-R in the brain and adipose tissue.
Results StD-ovariectomy-sedentary group had a greater
number of Ob-R positive neurons in lateral hypothalamic
nuclei than StD-Sh-sedentary group. There was no difference
in Ob-R positive neurons in arcuatus nuclei between
all groups. Ob-R distribution in the barrel cortex was higher
in HFD group than in StD group. Ob-R presence in perirenal
and subcutaneous fat was decreased in StD-ovariectomy
group.
Conclusion HFD and ovariectomy increased Ob-R distribution
in lateral hypothalamic nuclei, but there was no effect
on arcuatus nuclei. Our results are first to suggest that
HFD, ovariectomy, and physical activity affect Ob-R distribution
in the barrel cortex, which might be correlated with
the role of Ob-R in election of food in rats
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