Location of Repository

Induction of β-Glucosidases in Neurospora crassa

By Bruce M. Eberhart and Reta S. Beck

Abstract

The induction of β-glucosidases (EC 3.2.1.21) was studied in Neurospora crassa. Cellobiase was induced by cellobiose, but other inducers had little effect on this enzyme. Cellobiase activity was very low in all stages of the vegetative life cycle in the absence of di-β-glucoside inducer. Aryl-β-glucosidase was semiconstitutive at late stages of culture growth prior to conidiation. At early stages, aryl-β-glucosidase was induced by cellobiose, laminaribiose, and gentiobiose, and weakly induced by galactose, amino sugars, and aryl-β-glucosides. The induction properties of the β-glucosidases are compared with those of the other disaccharidases of Neurospora. The induction of β-glucosidases was inhibited by glucose, 2-deoxy-d-glucose, and sodium acetate. Sodium phosphate concentrations between 0.01 and 0.1 M stimulated induction of both enzymes, while concentrations above 0.1 M were inhibitory. The optimal condition for induction of both β-glucosidases was pH 6.0. Cellobiase induction was relatively more inhibited than aryl-β-glucosidase in the range of pH 6.0 to 8.0

Topics: Physiology and Metabolism
Year: 1973
OAI identifier: oai:pubmedcentral.nih.gov:246422
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefor we are unable to provide a PDF.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.