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The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro.

By S T Perry, M T Flaherty, M J Kelley, D L Clabough, S R Tronick, L Coggins, L Whetter, C R Lengel and F Fuller


Virulent, wild-type equine infectious anemia virus (EIAV) is restricted in one or more early steps in replication in equine skin fibroblast cells compared with cell culture-adapted virus, which is fully competent for replication in this cell type. We compared the sequences of wild-type EIAV and a full-length infectious proviral clone of the cell culture-adapted EIAV and found that the genomes were relatively well conserved with the exception of the envelope gene region, which showed extensive sequence differences. We therefore constructed several wild-type and cell culture-adapted virus chimeras to examine the role of the envelope gene in replication in different cell types in vitro. Unlike wild-type virus, which is restricted by an early event(s) for replication in equine dermis cells, the wild-type outer envelope gene chimeras are replication competent in this cell type. We conclude that even though there are extensive sequence differences between wild-type and cell culture-adapted viruses in the surface envelope gene region, this domain is not a determinant of the differing in vitro cell tropisms

Topics: Research Article
Year: 1992
OAI identifier: oai:pubmedcentral.nih.gov:241211
Provided by: PubMed Central
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