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Genetic mapping and transcriptional orientation of the fimD gene.

By C S Freitag and B I Eisenstein

Abstract

Expression of type 1 fimbriae in Escherichia coli exhibits phase variation whereby individual cells can alternate between states of organelle expression and nonexpression. Strains carrying fim-lac operon fusions in which lac operon expression is under the control of a fim promoter undergo Lac+ in equilibrium Lac- phase variation. We have determined the genetic map location and direction of transcription of a fim-lac operon fusion which was obtained by insertion of lac into a locus we have named fimD. We found the gene order to be as follows: valS fimD uxuA serB. The direction of transcription of fimD was found to be clockwise on the E. coli chromosome

Topics: Research Article
Year: 1983
OAI identifier: oai:pubmedcentral.nih.gov:217949
Provided by: PubMed Central
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