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Transformation of Streptococcus sanguis with monomeric pVA736 plasmid deoxyribonucleic acid.

By F L Macrina, K R Jones and R A Welch

Abstract

Monomeric and oligomeric forms of a 5.0 x 10(6)-dalton plasmid (conferring erythromycin resistance) were able to genetically transform naturally competent Streptococcus sanguis. Transformation with electrophoretically purified monomer was a second-order process, whereas transformation with a dye-buoyant density gradient-purified plasmid preparation followed one-hit kinetics

Topics: Research Article
Year: 1981
OAI identifier: oai:pubmedcentral.nih.gov:217034
Provided by: PubMed Central
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