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Transposon mutagenesis of Pseudomonas aeruginosa exoprotease genes.

By M J Stapleton, K S Jagger and R L Warren


Transposon Tn5 was used to generate protease-deficient insertion mutants of Pseudomonas aeruginosa. The presence of Tn5 in the chromosome of P. aeruginosa was demonstrated by transduction and DNA-DNA hybridization. The altered protease production and kanamycin resistance were cotransduced into a wild-type P. aeruginosa strain. A radiolabeled probe of Tn5 DNA hybridized to specific BamHI fragments isolated from the insertion mutants. Two independently isolated Tn5 insertion mutants had reduced protease production, partially impaired elastase activity, and no immunologically reactive alkaline protease

Topics: Research Article
Year: 1984
OAI identifier: oai:pubmedcentral.nih.gov:215121
Provided by: PubMed Central
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